Regulatory
Part:BBa_K1970004:Design
Designed by: Eivind Bøe Drejer Group: iGEM16_NTNU_Trondheim (2016-10-17)
Single deoxynucleotide ribonuclease that binds to and degrades CI repressor mRNA.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 337
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 615
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designing the sequence such that the binding affinity to the CI repressor mRNA was high enough was challenging.
Source
De novo DNA.